酶Acc I寡核苷酸序列GGTCGACC CGGTCGACCG CCGGTCGACCGGCACATGTG CCACATGTGG CCCACATGTGGGGGCGCGCC AGGCGCGCCT TTGGCGCGCCAACCCCGGGG CCCCCGGGGG TCCCCCGGGGGA切割率%2 hr20 hr0 0 0 0 00 >90 >90>90 >90 >9050 >90 >9000 >90 >90>90 >90 >90>90 >90 >90Afl IIIAsc IAva I酶 BamH I寡核苷酸序列切割率%2 hr20 hrBgl IIBssH IIBstE IIBstX ICGGATCCG CGGGATCCCG CGCGGATCCGCGCAGATCTG GAAGATCTTC GGAAGATCTTCCGGCGCGCC AGGCGCGCCT TTGGCGCGCCAAGGGT(A/T)ACCCAACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT10 >90 >900 75 250 0 5000 25 2525 >90 >900 >90 >900 0 >90100 50 >90酶寡核苷酸序列切割率%2 hr20 hr Cla IEcoR IHae IIIHind IIIKpn ICATCGATG GATCGATC CCATCGATGG CCCATCGATGGGGGAATTCC CGGAATTCCG CCGGAATTCCGGGGGGCCCC AGCGGCCGCT TTGCGGCCGCAACAAGCTTG CCAAGCTTGG CCCAAGCTTGGGGGGTACCC GGGGTACCCC CGGGGTACCCCG0 0 >90 50>90 >90 >90>90 >90 >900 0 100 >90 >900 0 >90 50>90 >90 >90>90 >90 >900 0 750 >90 >90酶 Mlu INco INde I寡核苷酸序列切割率%2 hr20 hrGACGCGTC CGACGCGTCGCCCATGGG CATGCCATGGCATGCCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC0 250 500 0 0 0 75 750 500 750 0 0 0 >90 >90Nhe IGGCTAGCC CGGCTAGCCG CTAGCTAGCTAG0 10 100 25 50酶Not I寡核苷酸序列TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAATGCATGCATGCA CCAATGCATTGGTTCTGCAGTTTTAATTAA GTTAATTAAC CCTTAATTAAGGGTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGGGCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT切割率%2 hr20 hr0 0 10 10 10 10 25 90 2510 >900 0 00 0 0 750 10 >90 >90 0>90>90 >900 25 >900 25 50 >900 10 >90 >90 0Nsi IPac IPme IPst I酶 Pvu I寡核苷酸序列切割率%2 hr20 hrSac ICCGATCGG ATCGATCGAT TCGCGATCGCGACGAGCTCG0 10 0100 25 1010Sac IISal ISca ISma ISpe IGCCGCGGC TCCCCGCGGGGAGTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGGCCATAGCGGCCGCGGAAGAGTACTC AAAAGTACTTTTCCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGAGACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG0 500 10 1010 750 0 10 >9010 10 0 00 >900 50 7525 7510 10 50 >90>90 >90 50 50酶 Sph I寡核苷酸序列切割率%2 hr20 hrStu IXba IXho IXma IGGCATGCC CATGCATGCATG ACATGCATGCATGTAAGGCCTT GAAGGCCTTC AAAAGGCCTTTTCTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAGCCTCGAGG CCCTCGAGGG CCGCTCGAGCGGCCCCGGGG CCCCCGGGGG CCCCCCGGGGGG 0 0 10>90 >90 >900 >90 75 750 10 100 25 50 0 25 50>90 >90 >900 >90 >90 >900 25 750 75 >90 TCCCCCCGGGGGGA>90>90
